Biology › Enzymes and metabolic control

Enzymes and metabolic control

Unit B03 · 4 lessons.

Notes

Illustrated notes for each topic in this unit, with worked examples and a checklist of objectives.

• Enzyme action and specificity
  Enzymes as globular protein catalysts, activation energy and what lowering it does and does not change, the active site and the enzyme-substrate…
• Factors affecting enzyme rate: temperature, pH and concentration
  Initial rate and why the rate of an enzyme-catalysed reaction falls during a run, the effect of temperature including Q10 and denaturation, the…
• Enzyme inhibition and metabolic control
  Competitive and non-competitive inhibition and their effects on Vmax and Km, reversible against irreversible inhibitors, named examples including…
• Cofactors, coenzymes and where enzymes work
  Cofactor ions, coenzymes and prosthetic groups, with chloride for amylase, NAD, coenzyme A and haem as the named examples.

Practical work

The practical capabilities this unit's work belongs to, each with its techniques, its calculations and the errors that cost marks.

• Measuring the rate of an enzyme-controlled reaction